Fig. 3.

CLE enhances the formation of eNOS dimer without altering expression levels of arginase II and eNOS. Protein levels of arginase II and eNOS were analyzed after incubation with CLE (18 hours, 20 µg/ml). Arginase II and eNOS protein levels were not significantly changed by CLE treatment (A, n=3). CLE incubation (20 µg/ml, 6 hours), however, induced eNOS dimerization, as detected by low-temperature SDS-PAGE and Western blot analysis (B). The dimer to monomer ratio of eNOS was shown in the bar graph from 4 independent experiments (* vs. untreated, p<0.01, n=4). Boiled samples were used as a control.