Fig. 2.

Contribution of H₂O₂-induced reactive oxygen species (ROS) to cytotoxicity in GS28 siRNA-transfected cells. Contribution of H₂O₂-induced ROS to cytotoxicity in GS28 siRNA-transfected cells was examined. siRNA-transfected cells were treated with H₂O₂ for 24 h in the presence of BSO without or with pretreatment with 1 mM tempol and 50 µM deferoxamine (DFO). Cell viability was determined by an MTT reduction assay. The data represent the mean±SEM of 3 independent experiments. ^*p<0.05 versus the values in GS28 siRNA-transfected cells without pretreatment.