Fig. 7.

H₂O₂-induced necroptotic cell death via activations of p38 MAPK in GS28 siRNA-transfected cells. (A) Pattern of H₂O₂-induced cell death was examined in GS28 siRNA-transfected cells without or with cointroduction of p38 siRNA. siRNA-transfected cells were treated with H₂O₂ for 3 h in the presence of BSO without or with pretreatment with 40 µM necrostatin-1 (Nec-1). The cells were stained with annexin V and propidium iodide (PI) and analyzed by flow cytometry, as described in Materials and Methods. (B) H₂O₂-induced cell death was examined in GS28 siRNA-transfected cells without or with pretreatment with 40 µM Nec-1. The cells were treated with H₂O₂ for 24 h in the presence of BSO. Cell viability was determined by an MTT reduction assay. The data represent the mean±SEM of 3 independent experiments. ^*p<0.05 versus the values in the cells transfected with GS28 siRNA alone. (C) H₂O₂-induced necroptotosis via activations of p38 MAPK was examined in GS28 siRNA-transfected cells. siRNA-transfected cells were treated with 100 µM H₂O₂ for 3 h without or with pretreatment with 40 µM Nec-1. Phosphorylation of p38 was determined by immunoblot analysis. β-actin was used as a loading control.