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. 2000 May 1;105(9):1233–1241. doi: 10.1172/JCI7610

Figure 2.

Figure 2

EGF-R expression in NF1 tumor sections. Paraffin sections were stained with anti–EGF-R (b, d, and f) or anti-S100 (a, c, and e) or with both antibodies (g, h, and i). Visualization of single antibodies was with 3′3-diaminobenzidine HCl (DAB; brown) (a–f). Double labeling used nitroblue tetrazolium (NBT/BCIP; blue) for anti-S100, and DAB (brown) for anti–EGF-R (g–i). In a, b, and d–f, the counterstain is hematoxylin (blue); in c and g–i, the counterstain is nuclear fast red (pink). (a–d) Sections from MPNST. In a, the arrow points to a normal nerve within the tumor, containing S100+ cells, whereas the tumor matrix is S100–. An adjacent section from the same tumor in b shows that most cells are EGF-R+. Another MPNST contains S100+ cells (c) and is mostly negative for EGF-R (d). (e and f) Sections of normal human nerve (arrow points to perineurium). (g) A section of a cutaneous neurofibroma with EGF-R+/S100+ cells. (h) A section from a dissociated neurofibroma cell preparation. The arrow designates a group of cells double stained by anti-S100 and anti–EGF-R. (i) A section from a dissociated normal nerve preparation. No double-labeled cells are detected. a–g, bar: 34.2 μm; h and i, bar: 16.3 μm.