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. 2011 Aug;24(4):351–355. doi: 10.1089/vim.2010.0140

FIG. 1.

FIG. 1.

The effect of thymectomy on CMV-specific memory T cells. Thymectomized (open circles) or sham operated (solid circles) wild-type (wt) B6 mice were MCMV infected. (A) On day 100, mice were sacrificed and splenocyte numbers were determined. (B) Splenocytes were stained for CD4, CD8, and CD44 expression. (C, D, and E) Splenocytes were stimulated in vitro with the indicated MCMV orf-derived peptides, and virus-specific CD8 (C and D) or CD4 (E) T cells were identified by flow cytometry for expression of IFN-γ. Results are displayed as percentages (top row) or numbers (bottom row) of peptide-specific CD8 or CD4 T cells/spleen, and are shown as mean ± SD of 6 mice/group. (F) Mean numbers and SD of epitope-specific CD8 T-cells/spleen at day 100 in two different experiments. CD4 T-cell determinations were done once. Statistical analysis was performed using the two-way Mann-Whitney U test.