TABLE I.
Comparison of actin patch dynamics detected through fluorescently labeled actin and the length of filopodia in vitro relative to in the living spinal cord
in vitro | in the spinal cord | p value | |
---|---|---|---|
Rate of patch formation (patches/20 /µm/5 min) |
2.04±0.4 (9) | 2.08±N/Aa (10) | N/A |
Patch lifespan (sec) | 48±4.6 (71) | 41.4±1.8 (52) | p=0.16* |
Probability of emergence of filopodia (0–1) |
0.15 (71) | 0.17 (52) | p=0.8** |
Patch size (pixels2) |
22 (36) | 23 (30) | p=0.3*** |
Length of filopodia (µm) |
3.6 (73) | 2.9 (146) | p<0.01*** |
Mean+SEM (n) are shown for the rate and lifespan data.
SEM is not available for the rate of patch formation in the spinal cord due to the sampling method, see main text. Patch sizes and filopodial lengths were not normally distributed and the median (n) is shown.
Welch t-test,
X2 test,
Mann-Whitney test.