Figure 1. H₂O₂ activation of EGFR is ligand-independent.

Serum-starved A549 cells were left intact or incubated with 40 nM monoclonal antibody 225 (mAb 225) for 1 hr on ice. Cells were then exposed for 15 min. at 37°C to 100 ng/ml EGF or 1 U/ml GO in the presence or absence of the mAb 225, as indicated. Immuno-precipitation (IP) of EGFR from cell lysates was performed using the mAb 528 and immuno-blotting (IB) for total (EGFR) and Tyr-phosphorylated EGFR (p-EGFR) was carried out as described in “Material and Methods”.