Figure 5. EGFR directly interacts with c-Src under H₂O₂ and such interaction is not abolished by Src family kinase inhibitor PP1.

A549 cells were incubated (or not) with 5 µM PP1 for 45 min. and then treated (or not) for 15 min. with 100 ng/ml EGF or 30 min. 1 U/ml GO. A. EGFR was IPed from total cell lysates with the mAb 528 and IBed for Y416 phosphorylated c-Src (p-Src) and for total EGFR, as indicated. B. c-Src was IPed from total cell lysates and IBed for total c-Src and EGFR, as indicated.