Effects of a 2-week caloric restriction on body weights, plasma leptin concentrations, and systolic BPs in 12-week-old F1 animals (+/+, Tg/+, Ay/+, and Tg/+:Ay/+ mice). (a) Time course of body weights of +/+ (open circles), Tg/+ (filled circles), Ay/+ (open boxes), and Tg/+:Ay/+ (filled boxes) mice during the caloric restriction. (b) Time course of plasma leptin concentrations in +/+ (open circles), Tg/+ (filled circles), Ay/+ (open boxes), and Tg/+:Ay/+ (filled boxes) mice. (c) Time course of systolic BPs in +/+ (open circles), Tg/+ (filled circles), Ay/+ (open boxes), and Tg/+:Ay/+ (filled boxes) mice. DP < 0.05, EP < 0.01, and FP < 0.001 (Tg/+ mice compared with +/+ mice), and AP < 0.05, BP < 0.01, and CP < 0.001 (Tg/+:Ay/+ mice compared with Ay/+ mice) assessed by ANOVA with repeated measures analysis with Student’s t test at a given time.