Figure 2.

Expression of PAI-1 and PAI-2 is elevated in Cav-1-deficient fibroblasts of human breast cancer tissues. Paraffin-embedded sections of human breast cancer tissues lacking stromal Cav-1 were immunostained with antibodies directed against PAI-1 (A and B) and PAI-2 (C and D). Slides were counterstained with hematoxylin. Note that PAI-1 stained positive in both stromal and cancer cells, whereas PAI-2 expression is observed only in stromal cells. White arrows indicate Cav-1-deficient fibroblasts that stained positive for both PAI-1 and PAI-2. Images were captured at an original magnification of 20x and 40x. s, stromal cells; c, cancer cells.