Skip to main content
. 2011 Jun 15;10(12):2021–2034. doi: 10.4161/cc.10.12.16002

Figure 6.

Figure 6

Fibroblasts overexpressing PAI-1 or PAI-2 promote tumor growth in vivo. To determine whether overexpression of PAI-1 or PAI-2 can phenocopy the tumor-promoting effects of Cav-1-deficient fibroblasts, fibroblasts (control vs. those overexpressing PAI-1 or PAI-2) were co-injected with a human breast cancer cell line [MDA-MB-231 (GFP+) cells] (MDA) in the flanks of nude mice. After 3 weeks, the tumors that formed were harvested and subjected to a detailed analysis. Note that relative to controls, PAI-1 or PAI-2 expressing fibroblasts increased tumor mass by ∼3-to-4-fold (A) and tumor volume by ∼4-fold. (B) Results are represented as the mean ± SEM. An asterisk indicates that p ≤ 0.05 compared with controls. n = 10 flank injections for each experimental group. Ctrl, fibroblasts containing empty vector alone; PAI-1, fibroblasts overexpressing PAI-1; PAI-2, fibroblasts overexpressing PAI-2; MDA, MDA-MB-231 (GFP+).