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. 2011 Jun 15;10(12):2021–2034. doi: 10.4161/cc.10.12.16002

Figure 7.

Figure 7

Fibroblasts overexpressing PAI-1 or PAI-2 do not affect tumor angiogenesis. Paraffin-embedded tumor sections from MDA-MB-231 cells (grown the presence of control fibroblasts or fibroblasts overexpressing PAI-1 or PAI-2) were immunostained with anti-CD31 antibodies and vessel density was determined. (A) Representative images for CD-31 immuno-staining are shown. Slides were counterstained with hematoxylin. Images were captured at 20x. (B) Quantification of the CD-31-positive vessels. Note that no significant differences in vessel density were observed. Results are represented as the mean ± SEM; n.s., not significant. (C) Immunhistochemical analysis of fibronectin. Note that MDA-MB-231 cells grown in the presence of fibroblasts overexpressing PAI-1 exhibit increased deposition of fibronectin in the stromal compartment. Images were captured at 20x. Ctrl, fibroblasts containing empty vector alone; PAI-1, fibroblasts overexpressing PAI-1; PAI-2, fibroblasts overexpressing PAI-2; MDA, MDA-MB-231 (GFP+).