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. 2011 Aug 10;6(8):e23365. doi: 10.1371/journal.pone.0023365

Figure 6. Absence of hepatic ER stress under n-3 PUFA depletion.

Figure 6

Mice were fed a control (CT; n = 6) or n-3 PUFA-depleted (DEF; n = 7) diet for 3 months. The hepatic mRNA content of glucose-regulated protein 78 (GRP78), glucose-regulated protein 94 (GRP94), protein disulfide isomerise (PDI), C/EBP homologous protein (CHOP), ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), unspliced (uXBP-1) and spliced (sXBP-1) X-box binding protein-1 is shown from fasted CT and DEF mice. The qPCR results were analysed according to the 2−ΔΔ ct method and were normalised to RPL-19 mRNA (A). Immunoblot analysis of GRP78, PDI, GRP94 and total and phosphorylated eukaryotic translation initiation factor 2α (eIF2α) (B) in the livers from fasted CT and DEF mice. Quantification of immunoblots is shown below. Data are the mean ± SEM. *: mean values significantly different (P<0.05, Student's t-test).