Figure 3.
Two-day Chrysin treatment on ATC cells caused an increase in the ratio of Bax to Bcl-2 in whole cell lysates. HTH7 (A) and KAT18 cells were treated with Chrysin for 2 days, and whole cell lysates were analyzed by Western blotting. To quantify the protein expression levels and ratio of Bax, Bcl-2, the bands on the X-ray films were scanned and the intensity of the bands was measured using ImageJ (ImageJ 1.43u, National Institute of Health). An increase in the ratio of intensity of Bax over Bcl-2 with Chrysin treatment was detected.