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. 2011 Aug 10;6(8):e23361. doi: 10.1371/journal.pone.0023361

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Choudhury et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Interaction between GmGβ (in pDEST32) and GmGγ (in pDEST22) proteins was determined using yeast-2 hybrid-based colorimetric assay. The assays were performed in triplicates and data were averaged. Error bars represent the standard error of the mean. Two biological replicates of the experiment were performed with similar results. (A) Interaction between GmGβ proteins and group I GmGγ proteins. (B) Interaction between GmGβ proteins and group II GmGγ proteins. (C) Interaction between GmGβ proteins and group III GmGγ proteins. Strong, weak and -ve refer to the interaction strength between RalGDS-wt-pDEST32 with Krev1-pDEST22, RalGDS-m1-pDEST32 with Krev1-pDEST22 and RalGDS-m2-pDEST32 with Krev1-pDEST22, respectively The controls are provided with the ProQuest two hybrid system (Invitrogen).