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. 2011 Aug 10;6(8):e23361. doi: 10.1371/journal.pone.0023361

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Choudhury et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Interaction between different members of group III GmGγ proteins was determined using yeast-2 hybrid-based growth and colorimetric assay. The assays were performed in triplicates and data were averaged. Error bars represent the standard error of the mean. Two biological replicates of the experiment were performed with similar results. Inset shows growth of yeast colonies on media lacking Leu and Trp but containing 50 µM 3-AT. Strong, weak and -ve refer to the interaction strength between RalGDS-wt-pDEST32 with Krev1-pDEST22, RalGDS-m1-pDEST32 with Krev1-pDEST22 and RalGDS-m2-pDEST32 with Krev1-pDEST22, respectively.