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. 2011 Aug 10;6(8):e23474. doi: 10.1371/journal.pone.0023474

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Cervelli et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) Low M_r DNA from a URA3⁺LEU2⁺ clone derived from co-transformation of pAAVRepURA with plasmid pG.Rep68, was digested with DpnI (loaded in the lane 2), MboI (loaded in the lane 3) , S1 nuclease (in the lane 4) and was analyzed on Southern Blot using URA3 probe (A) or ITR probe (B). (C) Southern blot analysis of low M_r of two URA3⁺LEU2⁺ clone derived from co-transformation of pAAVRepURA with plasmid pG.Rep68 was digested with Mung Bean nuclease (in the lane lane 2 and 4) and compared with the not digested DNA (lane 1, 3). DNA was detected using the URA3 probe. The arrows indicate the ssDNA which disappeared after digestion with S1 and Mung Bean nuclease.