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. 2011 Aug 10;6(8):e23560. doi: 10.1371/journal.pone.0023560

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This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Vero E6 cells were infected with Andes virus (m.o.i. = 0.01) and cultured in the presence of varying concentrations of ribavirin. (A) Supernatants collected at 3, 5 and 7 days post-infection were titered using a focus forming unit (FFU) assay. Day 1 supernatants were also collected, though titers were below the limit of detection of the focus assay. Data represent the average of three measurements. Error bars represent the standard error of the mean. (B–C) Andes virus RNA concentrations in supernatant (B) and Vero E6 cells (C) were determined using quantitative real-time RT-PCR targeting the nucleoprotein coding region. Error bars represent the standard error of the mean. (D) Andes virus nucleoprotein expression levels in infected Vero E6 cells cultured in the presence of ribavirin for 7 days post-infection. * p<0.05, ** p<0.01,*** p<0.0001.