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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Int J Cancer. 2011 Mar 29;129(11):2621–2631. doi: 10.1002/ijc.25939

Figure 4. Role of miR-328 in NSCLC.

Figure 4

(A) Migration of NSCLC cells. Graph showing migration of GFP-empty vector (Control) and miR-328 over-expressing cells for cell line (i) A549 and (ii) H1703 in modified microwell Boyden chamber assay. Both cell lines demonstrated significant increase in cell migration in miR-328 over-expression as compared to Control [p = 0.014 (A549) and 0.0006 (H1703)]. Plotted values are mean ± standard deviation of triplicate determinations. HPF: High Power Fields. (B) siRNA mediated knockdown of PRKCA. A549-328 cells were treated with specific PRKCA siRNAs (siPRKCA_1 or siPRKCA_2) or non-silencing control (NS) along with an Untreated (UT) control. Seventy-two hours later protein lysates were prepared and Western blot was performed. The results show successful knockdown of PRKCA protein expression. GAPDH was used as a loading control. (C) Effect of PRKCA knockdown on the migration of NSCLC cells. Graph showing migration of A549-328 cells vs. PRKCA silenced A549-328 cells in modified microwell Boyden chamber assay. PRKCA silencing in A549-328 cells demonstrated significant reduction in cell migration as compared to Untreated as well as Non-silencing control [*p < 0.005; #p<0.0001]. Plotted values are mean ± standard deviation of triplicate determinations. HPF: High Power Fields