Table 2.

Effects of high concentrations of Zn²⁺ and selenite on mitochondrial transmembrane potential of AS-30D cells monitored by flow cytometry after staining the cells with the lipophilic cationic probe JC-1.

Time	50 μM Se	250 μM Zn	500 μM Zn
3 h	67 ± 11*	55 ± 9*	46 ± 2*
24 h	14 ± 3*	13 ± 1*	12 ± 4*

The percentage of cells with red JC-1 fluorescence, reflecting high ΔΨ_mito (expressed in % to the corresponding control), is presented; the percentage of cells with high ΔΨ_mito in untreated control is accepted as 100%. Mean ±SE; n = 3 independent experiments; *P < 0.05 compared to untreated control.