Table 4.
Time-dependent effects of high concentrations of Zn2+ and selenite on ROS formation by AS-30D cells measured by flow cytometry using DCFH2-DA as ROS-sensitive probe.
| Medium | Time | DCF fluorescence (arbitrary units) | |||
|---|---|---|---|---|---|
| Control | 50 μM Se | 250 μM Zn | 500 μM Zn | ||
| PBS | 50 min | 32.8 ± 6.3 | 90.9 ± 3.1* | 38.5 ± 2.2 | 29.2 ± 9.5 |
| RPMI | 3 h | 21.1 ± 1.1 | 75.6 ± 2.5* | 31.6 ± 1.5* | 32.1 ± 1.8* |
| 24 h | 25.8 ± 4.3 | 4.2 ± 0.8* | 12.0 ± 1.0* | n.d. | |
| 48 h | 21.7 ± 4.7 | 7.3 ± 0.7* | 11.8 ± 0.5* | n.d. | |
The overall ROS production is shown as the geometric mean (±SE, n = 3) of total green fluorescence of the oxidation product of DCFH2. *P < 0.05 compared to untreated control; for other details, see Section 2; n.d., not determined.