Fig. 2.

Deficiency of insulin secretion caused by STZ prevents the HFD-induced ectopic fat accumulation. A: plasma levels of free fatty acids (FFA). Triglyceride contents in the liver (B) and gastrocnemius (C) of the mice described in Fig. 1 were measured and normalized to the protein level. Expression of key genes involved in fatty acid oxidation (Cpt1, Acadm, Acdal, and Acdavl) and lipogenesis were evaluated by real-time PCR in the liver (D) and gastrocnemius (E) of the mice described in Fig. 1 and normalized to 36B4. Results represent means ± SE. Veh: saline. *P < 0.05, **P < 0.01, and ***P < 0.001, Veh + HFD vs. ND. #P < 0.05, ##P < 0.01, and ###P < 0.001, STZ + HFD vs. Veh + HFD.