Table 1.
Primers used for real-time PCR
| Gene Name (Protein Name) | Forward (5′→3′) | Reverse (5′→3′) |
|---|---|---|
| Cpt1a (liver, CPT I) | GACTCCGCTCGCTCATTC | TCTGCCATCTTGAGTGGTGA |
| Cpt1b (muscle, M-CPT I) | TGCCTTTACATCGTCTCCAA | GGCTCCAGGGTTCAGAAAGT |
| Acadm (MCAD) | TGTCGAACACAACACTCGAAA | CTGCTGTTCCGTCAACTCAA |
| Acadl (LCAD) | TGGGGACTTGCTCTCAACA | GGCCTGTGCAATTGGAGTA |
| Acadvl (VLCAD) | GGTGGTTTGGGCCTCTCTA | GGGTAACGCTAACACCAAGG |
| Srebf1 (SREBP-1) | GGTTTTGAACGACATCGAAGA | CGGGAAGTCACTGTCTTGGT |
| Fasn (FAS) | CCAAATCCAACATGGGACA | TGCTCCAGGGATAACAGCA |
| Srebf2 (SREBP2) | ACCTAGACCTCGCCAAAGGT | GCACGGATAAGCAGGTTTGT |
| Hmgcr (HMG-CoAR) | TGCGTAAGCGCAGTTCCT | TTGTAGCCTCACAGTCCTTGG |
| Cs (citrate synthase) | CCATCCATAGTGACCATGAG | CTTTGCCAACTTCCTTCTGT |
| Gpx1(glutathione reductase 1) | GTGAGCCTGGGCTCCCTGCG | ACTTGAGGGAATTCAGAATC |
| Gsr (glutathione reductase) | GGGTGGCACTTGCGTGAATG | TTCAGGCGGCTCACATAGGC |
| Hhmox1 (HO-1) | CTATGTAAAGCGTCTCCA | GTCTTTGTGTTCCTCTGTC |
| Sod1 (Cu/Zn-SOD) | CAAGCGGTGAACCAGTTGTG | TGAGGTCCTGCACTGGTAC |
| Sod2 (MnSOD) | TCATGCAGCTGCACCACAGC | CCATTGAACTTCAGTGCAGG |
| Acsl1 (ACSL1) | AAAGATGGCTGGTTACACACG | CGATAATCTTCAAGGTGCCATT |
| Acsl5 (ACSL5) | ACAACCAGTCTGTGGGGATT | GGTCATTGTTCTTCTGGAAAGC |
| 36B4 | GCAGACAACGTGGGCTCCAAGCAGAT | GGTCCTCCTTGGTGAACACGAAGCCC |
CPT I, carnitine palmitoyltransferase I; M-CPT I, muscle CPT I; MCAD, medium-chain acyl-CoA dehydrogenase; LCAD, long-chain acyl-CoA dehydrogenase; VLCAD, very long-chain acyl-CoA dehydrogenase; SREBP-1 and -2, sterol regulatory element-binding protein-1 and -2, respectively; FAS, fatty acyl synthase; HMG-CoAR, hydroxymethylglutaryl-coenzyme A reductase; Cs, catalase; Gpx1, glutiathone peroxidase 1; Gsr, glutathione reductase; ACSL, acyl-CoA synthetases.