Fig. 3.
Lymphocyte function-associated antigen-1 (LFA-1) expression in monocytes. A: total protein expression. Human U937 monocytes were plated at 1 × 106 cells/ml and treated with AA (0–10 mM) or BHB (0–12 mM) for 24 h. Protein expression of CD11a and β-actin was determined by Western blotting by loading 10 μg of protein extract into an 8% gel. Bands were quantified using image J software and expressed per actin. Left: U937 monocytes. Right: THP-1 monocytes. B: surface expression of LFA-1. Following treatments, surface expression was determined by flow cytometry as described in Flow cytometry. Data are expressed as %CD11a + cells from the parent/gated population. C: mean fluorescence intensity (MFI) of 10,000 events. Left: U937 monocytes. Right: THP-1 monocytes. Values are means ± SE; n = 3.