Fig. 4.

LFA-1 affinity. Human THP-1 monocytes were plated at 1 × 10⁶ cells/ml and treated with AA (0–8 mM) or BHB (0–10 mM) for 24 h. mAb24 was added directly to the sample and incubated for 1 h prior to the end of the experiment to minimize loss of binding due to possible conformation changes by mechanical and physical stress during sample preparation. Following this, samples were processed as described in Flow cytometry. Values are means ± SE; n = 3.