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Chemokine secretion in ECs. Confluent monolayers of HUVECs were treated with AA (0–8 mM) or BHB (0–8 mM) for 24 h. Monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) levels were determined by assaying the supernatant using an ELISA kit. LPS (4 μg/ml) was used as a positive control. Data are representative of 4 different experiments. Values are means ± SE; n = 4.
