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. 2011 Aug 9;2011:679624. doi: 10.4061/2011/679624

Table 3.

Maximum specific activity (U/mg of protein) and the total units produced at the time point (hours) where the highest specific activity was found for each of the 5 isolates that showed enzymatic induction. 30°C and 39°C are the temperatures of growth where supernatant samples were collected to conduct the enzymatic assays. LB indicates the complex medium used, and starch refers to the induction medium containing starch as the major carbon source, in addition to a trace of peptone. All data points are average of 3 replicates of growth curves and 3 replicates of enzyme assay per time point (equals to 9 replicates).

Strain 30°C 39°C
LB Starch LB Starch
U/mg Total units Time point U/mg Total units Time point U/mg Total units Time point U/mg Total units Time point
URX291 0.079 ± 0.007 4 6 0.022 ± 0.001 4.3 16 0.043 ± 0.002 5.9 18 0.114 ± 0.016 8.2 6
URX303 0.098 ± 0.002 6.8 18 0.015 ± 0.002 2.7 16 0.036 ± 0.011 4.5 16 0.278 ± 0.024 25.2 26
URX350 0.097 ± 0.007 6.5 16 0.022 ± 0.004 2.2 16 0.037 ± 0.001 4.7 16 0.134 ± 0.014 19.4 18
UED641 0.094 ± 0.005 6.1 16 0.029 ± 0.002 2.9 4 0.035 ± 0.001 4.4 16 0.120 ± 0.006 8.7 2
UED644 0.160 ± 0.027 9.2 24 0.037 ± 0.003 3.2 2 0.035 ± 0.001 4.3 16 0.230 ± 0.024 19.4 2