Fig. 7.

c-Fos expression in C11-MDCK cells. A: representative Western blot showing the effects of ATP, charybdotoxin, and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) on c-Fos and GAPDH content in C11-MDCK cells. B: effect of charybdotoxin and NPPB on c-Fos/GAPDH ratio in control and ATP-treated cells. Cells were incubated in medium A containing 100 μM 6,N,N′-diethyl-β,γ-dibromomethylene-d-adenosine-5-triphosphate (ARL 67156) ± 0.1 μM charybdotoxin or 100 μM NPPB for 10 min, and then 50 μM ATP or vehicle were added for the next 30 min. c-Fos/GAPDH ratio in the absence of ATP and other tested compounds was taken as 1.0. Shown are the means ± SE obtained in 4 experiments.