Table 1.

Rab27b subapical colocalization with proteins involved in SV trafficking

Rab3D
At rest	73.6 ± 2.0% (n = 4)
+CCH	39.0 ± 7.4% (n = 4)
Myosin 5C
At rest	58.3 ± 8.1% (n = 6)
+CCH	53.4 ± 6.1% (n = 4)
F-actin
At rest	41.4 ± 6.2% (n = 4)
+CCH	22.0 ± 2.0% (n = 4)

Fluorescent pixel colocalization of Rab27b with different markers in reconstituted lacrimal gland acinar cells was measured with the Zeiss enhanced colocalization tool in a region of interest defined as the luminal area within a radius equal to half of the maximal distance from the apical to basal membrane. More than four random fields of view per preparation (n) were quantified. Values are given as the percentage of total fluorescent pixels associated with each marker in the region of interest that colocalized with Rab27b. Errors represent SE. CCH, carbachol.