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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Exp Parasitol. 2011 Mar 21;132(1):2–6. doi: 10.1016/j.exppara.2011.03.004

Fig. 1. Worms from single-sex infections express higher levels of both SmMRP1 and SMDR2 RNA than do paired worms.

Fig. 1

qRT-PCR analysis (SYBR) of RNA extracted from S. mansoni 6–7 weeks post-infection. Parasites were perfused from mice infected with males and females (white bar), single-sex males (grey bar), or single-sex females (black bar). Primers were directed against SMDR2 or SmMRP1, and the reference gene was 18S RNA. (A) Relative SMDR2 RNA expression (n= 3) (B) Relative SmMRP1 RNA expression (n= 3–4). *, **, *** indicate P<0.05, P<0.01, and P<0.001, respectively, ANOVA.