Figure 2.

Detection of ECE-2 transcripts in wild-type mouse embryos by in situ hybridization analysis (a, b, and c) and RT-PCR (d). (a) Transverse section of E13.5 wild-type mouse embryos showing strong expression of ECE-2 in the anterior part of the neural tube (nt), dorsal root ganglia (drg), and bilateral sympathetic trunk (sym). RA, right atrium; LA, left atrium; RV, right ventricle; LV, left ventricle. (b) Higher magnification of a, showing the heart. Note that ECE-2 expression (arrows) is detected in the mesenchyme of the endocardial cushion (ecc). (c) The sense probe did not show any positive signal. (d) ECE-2 expression is detectable by RT-PCR in E12.5 and E13.5 mouse hearts.