Fig. 1.

Western blot analysis of Smad3 expression in kidneys of Smad3^+/+ mice 24 h after bilateral renal ischemia (IR) for 25 min or sham surgery (Sham). Each lane represents protein extracted from a single kidney of an individual mouse. Equivalency of protein loading was assessed by immunoblotting for β-actin, and individual and mean standardized densitometric values are provided below the Western blot analysis. Smad3 expression was significantly higher in mice subjected to IR compared with mice subjected to Sham (P < 0.05).