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Western blot analysis of Smad3 expression in kidneys of Smad3+/+ and Smad3−/− mice 24 h after IR for 25 min or Sham. Each lane represents protein extracted from a single kidney of an individual mouse. Equivalency of protein loading was assessed by immunoblotting for β-actin, and individual and mean standardized densitometric values are provided below the Western blot analysis. No expression of Smad3 occurred in Smad3−/− mice.
