Fig. 6.

Semiquantitative assessment of necrosis in the kidney 24 h after IR for either 22.5 or 25 min in duration in Smad3^+/+ and Smad3^−/− mice. Shown are blinded analyses of the percentage of tubules exhibiting evidence of epithelial cell necrosis (A) and extension of cortical necrosis (B), the latter determined by the mean percentage of the distance from the corticomedullary junction to the surface of the kidney in which necrosis was present. No necrosis was observed in the kidneys of Smad3^+/+ and Smad3^−/− mice subjected to Sham (data not shown). *P < 0.05, Smad3^−/− vs. Smad3^+/+ mice subjected to respective IR duration.