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Renal expression of ET-1 mRNA 24 h after IR for either 22.5 or 25 min in duration and after Sham in Smad3+/+ and Smad3−/− mice. ET-1 mRNA expression was determined by quantitative real-time RT-PCR and standardized for 18S rRNA;. n = 7–8/group. *P < 0.05, Smad3−/− vs. Smad3+/+ mice subjected to respective IR duration.
