Table 1.
mRNA expression assessed by quantitative real-time RT-PCR
| Ischemia-Reperfusion |
||||||
|---|---|---|---|---|---|---|
| Sham |
22.5 min |
25 min |
||||
| Smad3+/+ | Smad3−/− | Smad3+/+ | Smad3−/− | Smad3+/+ | Smad3−/− | |
| HO-1 | 7.9 ± 1.1 | 11.9 ± 3.7 | 57.1 ± 11.9 | 35.1 ± 6.5 | 62.8 ± 8.3 | 47.9 ± 11.0 |
| MCP-1 | 5.9 ± 1.1 | 5.8 ± 0.7 | 62.5 ± 5.5 | 49.6 ± 6.7 | 55.6 ± 7.1 | 53.0 ± 6.1 |
| TNF-α | 5.6 ± 1.1 | 8.1 ± 1.8 | 63.0 ± 6.0 | 53.4 ± 6.7 | 51.8 ± 4.9 | 44.5 ± 6.9 |
Values are means ± SE and are the results of relative quantification performed against a standard curve constructed for each mRNA target, normalized for expression of 18S rRNA and expressed in arbitrary units; n = 7–8 for all groups. HO-1, heme oxygenase-1.