Representative cystometrogram recordings using continuous intravesical infusion of saline in conscious rats with an open outlet from a control (no CYP treatment) rat before and after blocking of AKT phosphorylation with intravesical deguelin instillation (1.0 μg/10 μl). A and B: bladder function before deguelin treatment (pre deguelin; A) and after deguelin treatment (post deguelin; B) in a control rat with continuous intravesical infusion of saline. Bladder function recordings in A and B are from the same rat. Infused volume (μl, top), bladder pressure (cmH2O, middle), and void volume (μl, bottom) before (A) and after (B) deguelin treatment are shown. s, Seconds.