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Western blot showing the effect of increasing concentrations of NHS-SS-biotin (NHS-B), maleimide-PEO2-biotin (MB), and MTSEA-B on precipitation of hOAT1 and hOAT3. The concentrations of NHS-B (0.01–1.0 mg/ml), MB (0.1–2.0 mg/ml), and MTSEA-B (0.1–1.0 mg/ml) used in the surface biotinylation reactions are shown.
