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. 2011 May 4;301(2):F378–F386. doi: 10.1152/ajprenal.00735.2010

Fig. 7.

Fig. 7.

A: representative Western blots showing the interaction of NHS-B, MB, and MTSEA-B with wild-type hOAT1 and the various mutant constructs. The precipitation of hOAT3 by these reagents is shown for comparison. B: densitometry analysis from several different Western blots (n = 2–4) examining relative cysteine accessibility in wild-type hOAT1, the mutant constructs of hOAT1, and hOAT3. Immunoreactivity corresponding to precipitation of the respective transport protein (PPT) with NHS-B was considered an appropriate measure of total hOAT1 (wild-type and mutant constructs) or hOAT3 expressed at the plasma membrane (5, 23) and thus was used to control for differences in surface expression. Immunoreactivity corresponding to precipitation with either MB or MTSEA-B was divided by immunoreactivity corresponding to precipitation with NHS-B to estimate relative cysteine accessibility (ratio of thiol:NHS PPT). The data are presented as a percentage of control (wild-type hOAT1) ratios (MB:NHS-B PPT and MTSEA-B:NHS-B PPT). *Significantly different from wild-type hOAT1, P < 0.05, Student's t-test.