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. 2000 May 15;105(10):1437–1445. doi: 10.1172/JCI7656

Figure 3.

Figure 3

The effect of the mutant of IRS-1 lacking PI3K binding sites on insulin sensitivity of IRS-1–deficient mice. (a) Introduction of IRS-1wt or IRS-1Δp85 into the IRS-1–deficient mouse liver by adenovirus-mediated gene transfer. After insulin treatment, the liver was isolated and lysed as described. The lysates were immunoprecipitated (IP) with 4G10 (upper panel) or αIRS-1-CT (middle panel) and subjected to SDS-PAGE (7% gel), followed by Western blotting (IB) with the same antibodies. The immunoprecipitates with 4G10 were also subjected to PI3K assay (lower panel). (b) ITT. ITT was performed against the indicated group of mice (eight mice in each group). Each bar represents ± SD. AP < 0.02 null IRS-1Δp85 versus null LacZ. BP < 0.01 IRS-1Δp85 versus null LacZ.