Fig. 6.

Gene expression changes. Mice were given the control or B₆-B₁₂-deficient diet for 2 wk followed by 5 days of 3% DSS. After euthanasia, colonic RNA was extracted for gene expression changes using TaqMan probes for real-time PCR (Con n = 10, Def n = 10, Con+3% n = 9, Def+3% n = 8). As expected, expression of TNF-α, inducible nitric oxide synthase (iNOS), and IL-10 were significantly increased in DSS mice compared with non-DSS controls (P < 0.05 DSS). Furthermore, TNF-α and iNOS were significantly lower in Def+3% compared with Con+3% (P < 0.05 Diet * DSS). IL-10 tended to be lower in the Def+3% compared with Con+3% (P = 0.09 Diet * DSS).