Fig. 7.

Changes in homocysteine and pyridoxal-phosphate (PLP) in plasma and tissues. Mice were given the control or B₆-B₁₂-deficient diet for 2 wk followed by 5 days of 3% DSS. A: Hcys levels were analyzed in plasma, colon, and liver (Con n = 10, Def n = 10, Con+3% n = 10, Def+3% n = 10). In the plasma, we saw a significant increase in Hcys in Def and Def+3% compared with Con and Con+3% (P < 0.05 Diet). In the liver, Hcys was significantly elevated in Def mice compared with Con (P < 0.05 Diet). In the colon, Hcys was similar in both Con and Def mice, and Def+3% was significantly higher than Con+3% (P < 0.05 Diet * DSS). B: PLP levels were analyzed in the plasma and colon (Con n = 10, Def n = 10, Con+3% n = 10, Def+3% n = 10). In both plasma and colon, we found a significant decrease in PLP in Def and Def+3% groups compared with controls (Con and Con+3%) (P < 0.05, Diet, DSS, Diet * DSS).