Table 1.
Oligonucleotides used in the TRP channel study
| Name | Sequences (5′-3′) | Tm | Size of cDNA, bp | Gene | Specificity |
|---|---|---|---|---|---|
| TRPC1-2 | CTGTGGATTATTGGGATGATTTGGTCA | 57 | 138/3611 | TRPC1 | M/H/MK |
| TRPC1-2r | CACTTTGAGGGCAAAGGTTGCCAA | 57 | TRPC1 | M/H/MK | |
| TRPC2-1 | CTCCTCCTGGCTGGGCTTG | 58 | 159/770 | TRPC2 | M/H |
| TRPC2-1r | GGTGAAGCTGAGCATGCTGGTG | 59 | Trpc2 | M/H | |
| TRPC3-2 | CAGCATTCTCAATCAGCCAACACG | 57 | 157/13058 | TRPC3 | M/H/MK |
| TRPC3-2r | AAGTTCATAACGAAGGCTGGAGATATC | 57 | TRPC3 | M/H/MK | |
| TRPC4-1 | CTACCTGATAGCTCCCAAAAGCC | 57 | 152/152 | TRPC4 | M/H/MK |
| TRPC4-1r | GACCTTGCCTGTTCAAGTCTGAC | 57 | TRPC4 | M/H/MK | |
| TRPC5-3 | CATCTCCCTGGTAGTGCTGCTG | 59 | 134/23393 | TRPC5 | M/H/MK |
| TRPC5-3r | CACCTTCATCAAAGTAACTCATCCAGAG | 58 | TRPC5 | M/H/MK | |
| TRPC5-1 | GGATTTTGCAATGAACTCCCTCTACC | 58 | 146/5557 | TRPC5 | M/H |
| TRPC5-1r | ATGTTGGATATTGCGAAGAGTGCTTC | 57 | TRPC5 | M/H | |
| TRPC6-2 | GTGGTCCTTGCTGTTGCCATTG | 57 | 175/9469 | TRPC6 | M/H/MK |
| TRPC6-2r | CTTCAAATCTGTCAGCTGCATTCATGAC | 58 | TRPC6 | M/H/MK | |
| TRPC7-2 | CTCATAATGAGAATCAAGATGTGCCTC | 57 | 136/1167 | TRPC7 | M/H/MK |
| TRPC7-2r | CAGAATTCCTCATGCCAGCCTG | 57 | TRPC7 | M/H/MK | |
| hTRPM1-1 | CGTGGATTGAAGCTCTTCCTTAGC | 57 | 185/23243 | TRPM1 | H/MK |
| hTRPM1-1r | CTTTCATTGATTTCTTCCAACCTCATTGAC | 58 | TRPM1 | H/MK | |
| hTRPM2-1 | CCTGCAGCAAGATCCTGAAGGA | 57 | 155/1574 | TRPM2 | H/MK |
| hTRPM2-1r | GAGCAGTTTCTGGGCTCTCTCTTC | 59 | TRPM2 | H/MK | |
| hTRPM3-1 | GAAGTGTTTGCGGACCAGATAGACC | 59 | 105/37825 | TRPM3 | H/MK |
| hTRPM3-1r | CACGATCCAAGCTCCTGTCTTGC | 59 | TRPM3 | H/MK | |
| hTRPM4-1 | CTGGATGAGCTGCGTTTGGCTG | 59 | TRPM4 | M/H/MK | |
| hTRPM4-1r | GAACTCAGGCCGGTCATTCAGCA | 59 | 141/1257 | TRPM4 | H/MK |
| TRPM5-1 | CAACTGGAACAAGTGTGACATGGTG | 58 | TRPM5 | M/H/MK | |
| hTRPM5-1r | CAGCGTGAACACCATGAAGTCCATG | 59 | 127/494 | TRPM5 | H/MK |
| TRPM6-1 | GAATCAACATGCAGGTCCATATGTGAC | 58 | 174/3159 | TRPM6 | M/H/MK |
| hTRPM6-1r | TGGCTCAAATACAATATCTCGAGCTAG | 57 | TRPM6 | H/MK | |
| hTRPM7-1 | GGTATGTGCGTTTGCTAGATTTTCTAGC | 58 | 180/5749 | TRPM7 | H/MK |
| hTRPM7-1r | GAGTCCAAGATGGTGCTTCATGAG | 57 | TRPM7 | H/MK | |
| TRPM8-1 | AAGCTTCTGCTGGAGTGGAACCAG | 59 | 126/4818 | TRPM8 | M/H/MK |
| TRPM8-1r | CTTGGGTCTGTCCTTTATGAGAGCC | 59 | TRPM8 | M/H/MK | |
| TRPV1-2 | GAGAGCAAGAACATCTGGAAGCTGC | 59 | 204/1502 | TRPV1 | M/H/MK |
| TRPV1-2r | GATGATGCCCACGTTGGTGTTCC | 59 | TRPV1 | M/H/MK | |
| hTRPV2-1 | GATCTGGCTGGACTTCCAGAGTAC | 59 | 110/2460 | TRPV2 | H/MK |
| TRPV2-1r | TTCAGCACAGCCTTCATCAGGCAC | 59 | TRPV2 | M/H/MK | |
| hTRPV3-1 | GCTGAAGAAGCGCATCTTTGCAGC | 59 | 135/910 | TRPV3 | H/Mk |
| TRPV3-1r | GTCAGCTTGTGCATGAGGAAGTCAG | 59 | TRPV3 | M/H/MK | |
| TRPV4-1 | GTGCTGGAGATCCTGGTGTACAAC | 59 | 136/2369 | TRPV4 | M/H/MK |
| TRPV4-1r | TAGGAGACCACGTTGATGTAGAAGG | 58 | TRPV4 | M/H/MK | |
| hTRPV5-1 | CTGCACATCGCTGTTGTGAACCAG | 59 | 159/466 | TRPV5 | H/MK |
| TRPV5-1r | CACACAGGCAGCAAAGGACAAAG | 57 | TRPV5 | M/H/MK | |
| TRPV6-1 | TGGTGCTGGTGACCATGGTGATG | 59 | 189/524 | TRPV6 | M/H/MK |
| TRPV6-1r | CCATCAGCCAGCAGAATCGCATC | 59 | TRPV6 | M/H/MK | |
| hB2M-1 | CTCTTTCTGGCCTGGAGGCTATC | 59 | B2M | H/MK | |
| hB2M-1r | GAAGTTGACTTACTGAAGAATGGAGAGCTCTCCATTCTTCAGTAAGTCAACTTC | 57 | 152 | B2M | H/MK |
Reference cDNA sequences for each gene in the NCBI were used to design oligonucleotides.
TRP, transient receptor potential; M, mouse; H, human; MK, monkey.