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DQ 65-79 does not affect the association of the p85 and p100 subunits of PI 3-kinase or the association of p85 with tyrosine phosphorylated proteins. (a) The p110-β subunit of PI 3-kinase was immunoprecipitated from peptide-treated cells stimulated with IL-2, and the blot was probed with an mAb specific for p85. (b) Anti-phosphotyrosine mAb was used to immunoprecipitate peptide-treated cells stimulated with IL-2. The blot was probed with anti-p85 mAb.
