A) Genital tubercle-enriched genes predicted to be expressed in UP epithelium
and GT (Col17a, Gja1, Dsc3) or UP mesenchyme and GT
(Syt13, Kcnab1, Osr2). Expression can also be seen in
preputial gland (Gja1), distal urethral epithelium
(Col17a, Gja1, Dsc3), surface epithelium of glans and
preputial swelling (Col17a, Gja1, Dsc3) and preputial
swelling mesenchyme (Kcnab1, Osr2). B) Genital
tubercle-restricted genes (Dlx6os1, Sostdc1, Cxcl14) showed
expression in preputial swelling epithelium (Sostdc1,
Cxcl14), distinct domains across the dorsal GT
(Dlx6os1, Sostdc1, Cxcl14) and distal genital tubercle
(glans) (Dlx6os1). Material is presented from left to right
as wholemounts of ventral GT, lateral GT (dorsal to the left side), dorsal
GT, and transverse and longitudinal sections through wholemount specimens.
di, distal; pr, proximal; d, dorsal; v, ventral; due, distal urethral
epithelium; up, urethral plate; upe, up epithelium; upm, up mesenchyme; ppg,
preputial gland; pps, preputial swelling.