Fig. 5.

Effects of p120 overexpression on cyclic stretch-induced gap formation. MLE-12 cells were transfected with p120 1A cDNA or empty vector. At 48 h posttransfection, cells were exposed to cyclic stretch (20%) for 2 h. A: p120 protein expression was determined by Western blot analysis. B: after fixation and permeabilization, cells were incubated with anti-p120-catenin primary antibody followed by Alexa-conjugated secondary antibody (green). The F-actin and nucleus were stained with Alexa 546 phalloidin (red) and DAPI (blue), respectively. Scale bars = 5 μm.