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. 2011 May 13;301(2):L161–L170. doi: 10.1152/ajplung.00073.2011

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Fig. 4. — A: simultaneous transfection of 293A cells with pcHA-PDE2A to express PDE2A and 10 separate green fluorescent protein (GFP)-tagged pcDNA-shRNAmiR^PDE2A constructs designed to suppress PDE2A expression. *pcDNA-shRNAmiR^PDE2A-921 was selected for Ad.PDE2A-shRNA [short hairpin RNA (shRNA) designed to selectively suppress PDE2A expression] construction. B: intratracheal dose-response effect of Ad.PDE2A-shRNA (derived from pcDNA-shRNAmiR^PDE2A-921) construct on mouse lung PDE2A expression measured 24 h after IT LPS administration. Ad.PDE2A-shRNA was administered 3 days before LPS (n = 2). Representative noncontiguous lanes from the same gel are shown. Values are means normalized to the effect of LPS without adenovirus. C: intratracheal dose-response effect of Ad.PDE2A-shRNA (derived from pcDNA-shRNAmiR^PDE2A-921) construct on mouse lung BAL protein, LDH activity, and total cell count 24 h after IT LPS administration. Ad.PDE2A-shRNA was administered 3 days before LPS (n = 2–3). Values are means ± SE normalized to the effect of LPS without adenovirus.