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The H4, R14 mutations in combination with GFP alter the cellular localization of AMSH when co-expressed with CHMP3.
(A) CHMP3-Flag and AMSH-HA.
(B) CHMP3-Flag and catalytic inactive GFP-AMSH(D348A).
(C) CHMP3-Flag and catalytic inactive GFP-AMSH(D348A-H4A-R14A).
(D) CHMP3-Flag and inactive AMSH-HA carrying the mutations H335Q and H337Q, which render it catalytic inactive as well as the N-terminal mutations H4A and R14A. The scale bar is 10 μM.
