Fig. 2.

Examples of immunoreactivity of sera obtained from PD patients with recombinant full-length synucleins. To confirm that protein bands detected by the PD serum GRFPD16 (a, top panel) represent α-synuclein, the membrane was re-probed with an anti-α-synuclein antibody (a, bottom panel, see also Materials and methods section). The right panels show the same membrane stained with Ponceau-S to visualize full-length recombinant synucleins (b, top panel) and probed with another PD serum GRFPD6, which has AAb against α- and β- but not γ-synuclein (b, bottom panel). Panel c shows examples of immunoblots probed with a control serum, sera from patients with sporadic form of PD (GRSPD) or sera from patients from PD families (GRFDP, designated by arrows in Fig. 1).