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SPR sensorgram of the binding of rat albumin to purified rat cubilin immobilized to a BIAcore sensor chip. The BIAevaluation software estimated Kd = 0.63 μM when fitting the curves to a one-site model. The on and off rates for binding of rat albumin were recorded by a flow of 5 μM albumin followed by flow of buffer alone. The SPR signal is displayed as the mass-equivalent RUs.
